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mouse anti gapdh  (Proteintech)


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    Structured Review

    Proteintech mouse anti gapdh
    Mouse Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+gapdh/GAPDH+Monoclonal+antibody/pmc13022619-94-0-6
    Average 98 stars, based on 10433 article reviews
    mouse anti gapdh - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Supplementation of glucomannans ameliorate high-fat diet-induced cognitive impairment via a gut microbiota-metabolite-glutamatergic synapse axis.
    Article Snippet: High-fat diets (HFDs) induce cognitive impairment and increase neurodegeneration risk.. Glucomannans (GMs) targeting the gut–brain axis are a promising dietary strategy, but the efficacy of specific glucomannans and their mechanisms remain unclear.. This study aimed to prepare GMs from various food sources, including Dendrobium officinale, konjac, and Aloe vera, and evaluate their potential to mitigate HFD-induced cognitive impairment.

    Western Blot:

    Article Title: Loss-of-function variants in ODAD1 disrupt ODA docking and induce actin cytoskeletal remodeling in primary ciliary dyskinesia
    Article Snippet: .. The primary antibodies used for western blotting included rabbit anti-ODAD1 (1:1000; Atlas Antibodies, cat# HPA042524), mouse anti-FLAG (1:1000; Sigma-Aldrich, cat# F1804), mouse anti-β-actin (1:2000; Proteintech, cat# 66009-1-Ig), mouse anti-V5 (1:1000; Invitrogen, cat# R96025 ), and mouse anti-GAPDH (1:2000; Proteintech, cat# 60004-1-Ig) antibodies. .. The following antibodies were used for immunofluorescence analyses: rabbit anti-ODAD1 (1:200; Atlas Antibodies, cat# HPA042524), mouse anti-acetylated α-tubulin (1:1000; Sigma-Aldrich, cat# T7451), rabbit anti-acetylated α-tubulin (1:1000; Cell Signaling Technology, cat# 5335), mouse anti-V5 (1:1000; Invitrogen, cat# R96025 ), mouse anti-CEP164 (1:50; Santa Cruz Biotechnology, cat# sc-515403), rabbit anti-VANGL1 (1:100; Cusabio, cat# CSB-PA848385ESR1HU), mouse anti-E-cadherin (1:200; Abcam, cat #ab231303), rabbit anti-SPEF2 (1:100; Atlas Antibodies, cat #HPA039606) and rabbit anti-CEP43 (Proteintech, cat# 11343-1-AP) antibodies.

    Article Title: Multiparity exacerbates Aβ accumulation and promotes cellular senescence in a mouse model of amyloidosis.
    Article Snippet: Proteins were extracted from cells after 3 days of treatment with 200 nM estradiol (E2) (Sigma Aldrich, E2758), 200 nM progesterone (P4) (MCE, HY-N0437), followed by BCA quantification (Thermo Fisher Scientific, A55864) and western blot analysis. .. Antibodies AR TIC LE IN PR ES S 8 The information of primary antibodies used was as follows: rabbit anti-IBA1 (FUJIFILM Wako Chemicals, 019-19741, PRID: AB_839504), goat anti-IBA1 (Novus, NB100-1028, PRID: AB_3148646, 1:500 for immunofluorescence (IF)), rabbit anti-GFAP (Abcam, ab7260, PRID: AB_305808), rat anti-CD68 (BIO-RAD, MCA1957, PRID: AB_3100585), rat anti-LAMP1 (DSHB, clone 1D4B, PRID: AB_2134500), rabbit anti-PU.1 (CST, 2258S, PRID: AB_2186909), rabbit anti-SYNAPTOPHYSIN (Abcam, ab16659, PRID: AB_443419), rabbit anti-PSD-95 (Invitrogen, 51-6900, PRID: AB_2533914), rabbit anti-BACE1 (CST, 5606S, PRID: AB_1903900), rabbit anti-Amyloid Precursor Protein, C-Terminal (Sigma-Aldrich, A8717, PRID: AB_258409), mouse anti-PSD-95 (1:1000; mouse monoclonal, NeuroMab, 75-028, PRID: AB_2292909), rabbit antiFOSB (1:1000; CST, 2251S, PRID: AB_2106903), rabbit anti-SYNAPTOPHYSIN (1:1000 for WB; Abclonal, A6344, PRID: AB_2766946), rabbit anti-BACE1 (rabbit monoclonal, CST, 5606S, PRID: AB_1903900, 1:1000 for WB, 1:500 for IF), chicken anti-MAP2 (1:5000, Abcam, ab5392, PRID: AB_2138153), rabbit anti-Beta-Actin (1:10000; mouse monoclonal; Proteintech, 66009- 1-Ig, PRID: AB_2687938), rabbit anti-β Tubulin (Proteintech, 10094-1-AP, PRID: AB_2210695, 1:5000 for WB) and mouse anti-GAPDH (ProteinTech, 60004-1-Ig, PRID: AB_2107436, 1:10000 for WB). .. The information of secondary antibodies used was as follows: donkey anti-rabbit IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-21206, PRID: AB_2535729), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A10042, PRID: AB_2534017), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 647 (Invitrogen, A-31573, PRID: AB_2536183), donkey anti-goat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-11055, PRID: AB_2534102), donkey anti-goat IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A-11057, PRID: AB_2534104), donkey anti-rat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A48269, PRID: AB_2893137), donkey anti chicken IgY AR TIC LE IN PR ES S 9 (H+L) highly cross adsorbed secondary antibody, Alexa FluorTM 488 (Invitrogen, A78948, PRID: AB_2921070), all diluted by 10% donkey serum in PBS 1:500 for IF.

    Immunofluorescence:

    Article Title: Multiparity exacerbates Aβ accumulation and promotes cellular senescence in a mouse model of amyloidosis.
    Article Snippet: Proteins were extracted from cells after 3 days of treatment with 200 nM estradiol (E2) (Sigma Aldrich, E2758), 200 nM progesterone (P4) (MCE, HY-N0437), followed by BCA quantification (Thermo Fisher Scientific, A55864) and western blot analysis. .. Antibodies AR TIC LE IN PR ES S 8 The information of primary antibodies used was as follows: rabbit anti-IBA1 (FUJIFILM Wako Chemicals, 019-19741, PRID: AB_839504), goat anti-IBA1 (Novus, NB100-1028, PRID: AB_3148646, 1:500 for immunofluorescence (IF)), rabbit anti-GFAP (Abcam, ab7260, PRID: AB_305808), rat anti-CD68 (BIO-RAD, MCA1957, PRID: AB_3100585), rat anti-LAMP1 (DSHB, clone 1D4B, PRID: AB_2134500), rabbit anti-PU.1 (CST, 2258S, PRID: AB_2186909), rabbit anti-SYNAPTOPHYSIN (Abcam, ab16659, PRID: AB_443419), rabbit anti-PSD-95 (Invitrogen, 51-6900, PRID: AB_2533914), rabbit anti-BACE1 (CST, 5606S, PRID: AB_1903900), rabbit anti-Amyloid Precursor Protein, C-Terminal (Sigma-Aldrich, A8717, PRID: AB_258409), mouse anti-PSD-95 (1:1000; mouse monoclonal, NeuroMab, 75-028, PRID: AB_2292909), rabbit antiFOSB (1:1000; CST, 2251S, PRID: AB_2106903), rabbit anti-SYNAPTOPHYSIN (1:1000 for WB; Abclonal, A6344, PRID: AB_2766946), rabbit anti-BACE1 (rabbit monoclonal, CST, 5606S, PRID: AB_1903900, 1:1000 for WB, 1:500 for IF), chicken anti-MAP2 (1:5000, Abcam, ab5392, PRID: AB_2138153), rabbit anti-Beta-Actin (1:10000; mouse monoclonal; Proteintech, 66009- 1-Ig, PRID: AB_2687938), rabbit anti-β Tubulin (Proteintech, 10094-1-AP, PRID: AB_2210695, 1:5000 for WB) and mouse anti-GAPDH (ProteinTech, 60004-1-Ig, PRID: AB_2107436, 1:10000 for WB). .. The information of secondary antibodies used was as follows: donkey anti-rabbit IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-21206, PRID: AB_2535729), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A10042, PRID: AB_2534017), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 647 (Invitrogen, A-31573, PRID: AB_2536183), donkey anti-goat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-11055, PRID: AB_2534102), donkey anti-goat IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A-11057, PRID: AB_2534104), donkey anti-rat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A48269, PRID: AB_2893137), donkey anti chicken IgY AR TIC LE IN PR ES S 9 (H+L) highly cross adsorbed secondary antibody, Alexa FluorTM 488 (Invitrogen, A78948, PRID: AB_2921070), all diluted by 10% donkey serum in PBS 1:500 for IF.

    Recombinant:

    Article Title: E3 ubiquitin ligase Stub1 enhances viral replication by promoting TBK1 degradation through molecular chaperone-mediated autophagy
    Article Snippet: Poly (dA: dT) (P0883-10UN) and antiFlag (M2) beads (M8823) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Mouse anti-GAPDH (60004-1-Ig), rabbit anti-TBK1 (28397-1-AP), recombinant human ubiquitin protein (Ag0260), recombinant human UBE1 protein (Ag8920), and recombinant human UBE2N protein (Ag0292) were purchased from Proteintech (Wuhan, China). .. Rabbit anti-phospho-TBK1 (5483), rabbit antiIRF3 (11904), and rabbit anti-phospho-IRF3 (29047) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Ubiquitin Proteomics:

    Article Title: E3 ubiquitin ligase Stub1 enhances viral replication by promoting TBK1 degradation through molecular chaperone-mediated autophagy
    Article Snippet: Poly (dA: dT) (P0883-10UN) and antiFlag (M2) beads (M8823) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Mouse anti-GAPDH (60004-1-Ig), rabbit anti-TBK1 (28397-1-AP), recombinant human ubiquitin protein (Ag0260), recombinant human UBE1 protein (Ag8920), and recombinant human UBE2N protein (Ag0292) were purchased from Proteintech (Wuhan, China). .. Rabbit anti-phospho-TBK1 (5483), rabbit antiIRF3 (11904), and rabbit anti-phospho-IRF3 (29047) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

    other:

    Article Title: Mechanosensory channels mediate ER Ca 2+ transients to trigger assembly of autophagosome initiation sites for degradation of ER subdomains.
    Article Snippet: Article Mechanosensory channels mediate ER Ca2+ transients to trigger assembly of autophagosome initiation sites for degradation of ER subdomains



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